所有图片(1)

C8946

α-糜蛋白酶来源于人类胰腺

lyophilized powder

所有图片(1)

CAS号:

9004-07-3

EC 号:

3.4.21.1 (BRENDA, IUBMB)

EC 号:

232-671-2

MDL编号:

MFCD01323069

NACRES:

NA.54

质量水平

200

生物来源

human pancreas

形式

lyophilized powder

分子量

25 kDa

包装

vial of ≥10 BTEE units

UniProt登记号

P17538

储存温度

−20°C

Gene Information

human ... CTRB1(1504)

一般描述

α-Chymotrypsin belongs to the trypsin family of serine proteases. It is secreted as an inactive precursor chymotrypsinogen to the intestine. The cleavage of peptide bond at amino acid 148 forms α-chymotrypsin. Chymotrypsinogen is encoded by the gene CTRB1 and is mapped to 16q23.1 in the human chromosome.

应用

α-Chymotrypsin from human pancreas has been used to test inhibition by N-alkyl isatins against mammalian serine proteases.

Human α-chymotrypsin has been used in a study to assess the quantitative structure-activity relationships for organophosphates binding to trypsin and chymotrypsin. Human α-chymotrypsin has also been used in a study to investigate the direct detection of native proteins in biological matrices using extractive electrospray ionization mass spectrometry.

生化/生理作用

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met) on the carboxyl end of the bond.

单位定义

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25°C.

分析说明

由 A1%/280 测定的蛋白质

象形图

GHS07,GHS08,GHS09

警示用语：

Danger

危险声明

H302 - H315 - H319 - H334 - H335 - H400

预防措施声明

P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 1

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

分析证书

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原产地证书 (CofO)

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Quotes and Ordering

Bulk Quote-Order Product

Parallel synthesis of isatin-based serine protease inhibitors

Shuttleworth SJ, et al.

Bioorganic & Medicinal Chemistry Letters, 10(22), 2501-2504 (2000)

Crystal structure of delta-chymotrypsin bound to a peptidyl chloromethyl ketone inhibitor

Mac Sweeney A, et al.

Acta Crystallographica Section D, Biological Crystallography, 56(3), 280-286 (2000)

Genome-wide association study identifies inversion in the CTRB1-CTRB2 locus to modify risk for alcoholic and non-alcoholic chronic pancreatitis

Rosendahl J, et al.

Gut, 67(10), 1855-1863 (2018)

Enhanced mass spectrometric mapping of the human GalNAc-type O-glycoproteome with SimpleCells.

Sergey Y Vakhrushev et al.

Molecular & cellular proteomics : MCP, 12(4), 932-944 (2013-02-13)

Characterizing protein GalNAc-type O-glycosylation has long been a major challenge, and as a result, our understanding of this glycoproteome is particularly poor. Recently, we presented a novel strategy for high throughput identification of O-GalNAc glycosites using zinc finger nuclease gene-engineered

Effect of limited enzymatic hydrolysis on linoleic acid binding properties of β-lactoglobulin.

Osvaldo E Sponton et al.

Food chemistry, 146, 577-582 (2013-11-02)

β-Lactoglobulin (BLG) is a member of lipocalin family, proteins with ability to bind small hydrophobic ligands, such as retinol, vitamins and fatty acids. Moreover, BLG is susceptible to protease action producing a wide range of polypeptides depending on the hydrolysis

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实验方案

Procedure for Enzymatic Assay of α-Chymotrypsin (EC 3.4.21.1)

Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.