Merck

C9080

Sigma-Aldrich

单克隆抗波形蛋白抗体

clone V9, purified immunoglobulin, buffered aqueous solution

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别名:
Monoclonal Anti-Vimentin
NACRES:
NA.41

生物来源

mouse

偶联物

CY3 conjugate

抗体形式

purified immunoglobulin

antibody product type

primary antibodies

克隆

V9, monoclonal

形式

buffered aqueous solution

分子量

antigen ~58 kDa

species reactivity

pig, canine, feline, hamster, rabbit, gerbil, monkey, bovine, chicken, human, horse, rat

technique(s)

direct immunofluorescence: 1:200 using cultured cells
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 using human tonsil
immunohistochemistry (frozen sections): suitable using human tonsil

同位素/亚型

IgG1

UniProt登记号

运输

wet ice

储存温度

2-8°C

target post-translational modification

unmodified

Gene Information

human ... VIM(7431)
rat ... Vim(81818)

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此商品
V6389V5255V2258
conjugate

CY3 conjugate

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

ascites fluid

clone

V9, monoclonal

clone

V9, monoclonal

clone

VIM-13.2, monoclonal

clone

LN-6, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

-

mol wt

antigen ~58 kDa

mol wt

antigen ~58 kDa

mol wt

-

mol wt

-

一般描述

Monoclonal Anti-Vimentin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Vimentin is expressed in breast myoepithelial cells, osteocytes, Langerhans cells of the skin, Schwann cells and astrocytes.
Vimentin is one of the five major groups of intermediate filaments with a molecular mass of 57 kDa. Intermediate filaments (IFs) with characteristic 10 nm diameter are a distinct class of molecularly heterogenous cytoskeletal filaments defined by ultrastructural, immunological and biochemical criteria. IFs differ significantly from the other cytoskeletal elements of the cell, namely microtubules and microfilaments and are components of most eukaryotic cells.

特异性

在免疫组织化学染色中,抗体将波形蛋白定位于成纤维细胞、内皮细胞、淋巴组织和黑色素细胞中。它也在肉瘤、淋巴瘤和黑色素瘤中染色波形蛋白。

免疫原

猪眼晶状体波形蛋白。

应用

Monoclonal Anti-Vimentin-Cy3 antibody produced in mouse has been used in:
  • immunofluorescence and confocal microscopy
  • cytoskeleton staining
  • double labeling experiments
  • immunohistochemical
  • immunocytochemical localozation

生化/生理作用

Cy3 Monoclonal Anti-Vimentin reacts with normal and pathological tissue of mesenchymally-derived cells, lens tissue and various cultured cells. It localizes vimentin in fibroblasts, lipocytes, endothelial cells, some lymphoid cells, melanocytes, macrophages, and chondrocytes. The antibody stains tumors derived from these cells, including sarcomas, most lymphomas, melanomas and their metastatic lesions. Co-expression of vimentin with keratin was described in most carcinomas of certain sites (e.g., kidney and thyroid) and certain carcinomas of other sites (e.g., breast).
Vimentin was found to be important for stabilizing the architecture of the cytoplasm. It was found, that activated macrophages secrete vimentin into the extracellular space in vitro.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

储存及稳定性

Store at 2-8 °C. Protect from prolonged exposure to light. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

法律信息

Cy is distributed under license from Amersham Biosciences Limited.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

10 - Combustible liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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