C9354

Sigma-Aldrich

Monoclonal Anti-COX II antibody produced in mouse

clone AS66, purified immunoglobulin, buffered aqueous solution

别名:
Anti-Cyclooxygenase II
MDL number:

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AS66, monoclonal

form

buffered aqueous solution

species reactivity

human

application(s)

flow cytometry: 1 μg/test
indirect ELISA: suitable
microarray: suitable
western blot: suitable

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... PTGS2(5743)

General description

COX-II is an isoform of COX enzyme with 604 amino acid residues that catalyzes the conversion of arachinodate to prostaglandin H2.

Immunogen

recombinant full length human COX II, 604 amino acids.

Application

Monoclonal Anti-COX II antibody produced in mouse is suitable for immunoblotting at a working concentration of 1 to 5 μg/mL, indirect ELISA, microarray and flow cytometry at 1 μg/test.

Biochem/physiol Actions

COX-II is a target of NSAID (non-steroidal anti-inflammatory drugs) such as aspirin. It is induced in migratory cells responding to pro-inflammatory stimuli and is an important mediator of inflammation. The prostaglandins produced by COX-II are responsible for the pain and swelling from inflammation. As this enzyme is involved in the production of inflammatory agents, it is the target of intense research and drug discovery activities. COX-II is expressed in new angiogenic endothelial cells, synoviocytes from rheumatoid arthritis patients. It is also markedly expressed in 85% to 90% of human colorectal adenocarcinomas.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 0.08% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

C Williams et al.
Annals of the New York Academy of Sciences, 889, 72-83 (2000-02-11)
Cyclooxygenase (COX), the key regulatory enzyme for prostaglandin synthesis is transcribed from two distinct genes. COX-1 is expressed constitutively in most tissues, and COX-2 is induced by a wide variety of stimuli and was initially identified as an immediate-early growth...
J Adams et al.
Journal of neurochemistry, 66(1), 6-13 (1996-01-01)
We have characterised the induction of the mitogen-inducible form of cyclooxygenase, COX-2, in the rat cerebral cortex in response to excitotoxin injection into the nucleus basalis. This model is associated with intense stimulation of the ascending pathway to the cerebral...
R N Dubois
Alimentary pharmacology & therapeutics, 14 Suppl 1, 64-67 (2000-05-12)
Use of nonsteroidal anti-inflammatory drugs such as aspirin, which are known to inhibit cyclooxygenase activity, reduces the relative risk of colorectal cancer in humans by 40-50%. Animal and human studies have shown a 50-80% reduction in tumour multiplicity following treatment...
G P O'Neill et al.
Molecular pharmacology, 45(2), 245-254 (1994-02-01)
Human prostaglandin G/H synthase (hPGHS)-1 and hPGHS-2, key enzymes in the formation of prostanoids from arachidonic acid, were expressed at high levels in COS-7 cells using a T7 RNA polymerase/vaccinia virus expression system. The open reading frame of hPGHS-2 cloned...
J R Vane et al.
Inflammation research : official journal of the European Histamine Research Society ... [et al.], 44(1), 1-10 (1995-01-01)
The discovery of a second cyclooxygenase has provided fresh impetus to the search for new anti-inflammatory drugs. The second enzyme is effectively absent from healthy tissues but its levels rise dramatically during inflammation. It can be induced in migratory cells...

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