Merck

BICR 6

05070501

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生物来源

human pharynx

生长模式

Adherent

核型

Aneuploid

形态学

Epithelial, keratinocyte

产品

Not specified

受体

Epidermal Growth Factor Receptor (EGFR)

technique(s)

cell culture | mammalian: suitable

相关疾病

cancer

运输

dry ice

储存温度

−196°C

细胞系来源

Hypopharynx squamous cell carcinoma

细胞系描述

Adherent keratinocyte cell line derived from a squamous cell carcinoma of the hypopharynx of a Caucasian male. Keratin and involucrin markers present. Known mutations: p53, p16 and p14ARF. Cells are tumourigenic in athymic mice. Epidermal Growth Factor Receptor (EGFR) present.

培养基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.

传代培养常规

Split sub-confluent cultures (70-80%) 1:10 - 1:50 using 0.05% trypsin/EDTA; 8% CO2; 37 C. Suggested seeding density 2 x 10,000 cells/cm2. Culture cells on a feeder layer of lethally-irradiated or mitomycin C-treated 3T3 feeder cells.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免责声明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

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