Merck

U-251 MG cell line human

09063001

别名:
U-251MG Cells, U251 Cells, U251N Cells

生物来源

human brain

生长模式

Adherent

核型

2n = 46

形态学

Pleomorphic/astrocytoid

产品

Contains GFAP positive cells

受体

PDGFR alpha and EGFR. See Nistér M. et al., (1988) & (1991)

technique(s)

cell culture | mammalian: suitable

相关疾病

cancer

运输

dry ice

储存温度

−196°C

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此商品
CB_12021502CB_12022001CB_12021503
U-251 MG cell line human 09063001

U-251 MG cell line human

U2A cell line human 12021502

U2A cell line human

293T cell line human 12022001

293T cell line human

U3A Cell line human 12021503

U3A Cell line human

growth mode

Adherent

growth mode

Adherent

growth mode

adherent

growth mode

-

karyotype

2n = 46

karyotype

-

karyotype

-

karyotype

-

morphology

Pleomorphic/astrocytoid

morphology

Epithelial

morphology

small slightly rounded cells growing in a dispersed manner

morphology

Epithelial

products

Contains GFAP positive cells

products

-

products

SV40 T antigen

products

-

receptors

PDGFR alpha and EGFR. See Nistér M. et al., (1988) & (1991)

receptors

-

receptors

-

receptors

-

细胞系来源

Human glioblastoma astrocytoma

细胞系描述

Derived from a malignant glioblastoma tumour by explant technique. U-251 was formerly distributed as U-373 MG (Sigma product no. 89081403) until short tandem repeat (STR)-PCR profiling confirmed identity with U-251. A new deposit of U-373 MG known as U-373 MG (Uppsala) is now available (Sigma product no. 08061901).Background to the identity query for the cell line U-373 MG: The American Type Culture Collection (ATCC) reported that their stock of U-373 MG had been shown to have differing genetic properties to stock from the originator′s laboratory, and to share similarities with another glioblastoma cell line, U-251. In light of this, ECACC undertook an investigation into the authenticity of its own stock of the U-373 MG cell line. ECACC found similar results to the ATCC i.e. the stock held as U-373 MG was found to be identical by STR-PCR profiling to U-251. The U-373 MG cell line listed under product no. 89081403 has been re-named as ′U-251 (formerly known as U-373 MG)′ and has the new Sigma product no. 09063001.

应用

U-251 MG cell line human has been used:
  • for the extraction of cancer stem cells from U-251 human glioblastoma cell line
  • to study the mechanism of Pax6 (paired box protein)-associated increase in expression of Dkk3 (Dickkopf 3)
  • to study the role of JARID1B (jumonji AT-rich interactive domain 1B) in the pathogenesis of glioma
  • to study the therapeutic efficacy of Irinophore C combined with temozolomide in glioblastoma tumor model

DNA图谱分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 10,11
D16S539: 12
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 16,18

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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