U3A Cell line human

12021503, human fibrosarcoma, Epithelial

• 别名: 2fTGH, 2fTGH-U3A
• UNSPSC代码: 41106514

属性

• 产品名称: U3A Cell line human, 12021503
• 生物来源: human fibrosarcoma
• 形态学: Epithelial
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系描述

The human sarcoma cell line HT 1080 (ECACC Catalogue number 85111505) was transfected with a vector encoding a selectable marker (guanine phosphoribosyltransferase) regulated by alpha interferon to create the 2fTGH cell line, enabling the selection of mutations in genes encoding components of the interferon signalling pathway. Chemical mutagenesis of the parent 2fTGH cell line enabled the isolation of a number of IFNg signalling mutants available from ECACC: U2A (12021502), U3A (12021503), U4A (12021504), U4C (12021505), U5A (12021506), U6A (12021507). Each contains a different mutation in the IFN signalling pathway. The 2fTGH panel provides a tool for the in vitro study and comparison of disrupted interferon signalling at multiple points across the IFN pathway. The U3A mutant cell line lacks expression of STAT1 gene product. U3A is unresponsive to interferon-alpha and interferon-gamma. U3A is partially defective in its response to double stranded RNA.

DNA图谱分析

STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 12
D13S317: 12,14
D16S539: 9,12
D5S818: 11,13
D7S820: 9,10
THO1: 6
TPOX: 8
vWA: 14,19

培养基

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS (F2442).

传代培养常规

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

其他说明

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引用

If use of this culture results in a scientific publication, it should be cited in the publication as: U3A (ECACC 12021503)

免责声明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.