HeLa Ohio

84121901

属性

• 生物来源: human cervix
• 生长模式: Adherent
• 核型: Aneuploid
• 形态学: Epithelial
• 产品: Not specified
• 受体: Not specified
• technique(s): cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human cervix carcinoma

细胞系描述

Derivative of parent HeLa line (Sigma catalogue no. 93021013).

应用

Tumourigenicity and virus studies: poliovirus 1 and Ad 3

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他说明

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