J774.2 Cell Line from mouse

BALB/C monocyte macrophage, 85011428

• 别名: J774.2 Cells

属性

• 生物来源: mouse blood
• 生长模式: Semi-adherent
• 核型: Not specified
• 产品: Not specified
• 受体: Immunoglobulin, complement
• technique(s): cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Mouse BALB/c monocyte macrophage

细胞系描述

Recloned from J774.1 original ascites and solid tumour. Produces IL-1. Cell line J774.2 was recloned from the original ascites and solid tumor J774.1, and produces interleukin-1 (IL-1).

应用

J774.2 Cell Line from mouse has been used:

• to study the effect of the growth hormone secretagogue peptide A233 on J774.2 cell line
• to study the ability of Mycobacterium tuberculosis clinical isolates to penetrate and grow inside the J774.2 cell line
• to study the effect of ivy leaf dry extract EA 575 on the lipopolysaccharide-induced release of interleukin-6 (IL-6) from J774.2 cell line

培养基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cells are semi-adherent i.e. some cells grow in suspension , some loosely attach to the surface and others flattened out and attach to the flask. They should be passaged by scraping. Cells should not be allowed to overgrow and become confluent as this can lead to a loss of the flattened adherent cell characteristic.

其他说明

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