RAJI Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85011429, human lymph, Lymphoblastoid

• 别名: GM04671 Cells, P1-Raji Cells
• UNSPSC代码: 41106514

属性

• 生物来源: human lymph
• 描述: Human Black, Burkitt′s lymphoma
• 包装: tube of 5 μg 85011429-DNA-5UG; pkg of vial of cells 85011429-1VL
• 生长模式: Suspension
• 核型: 2n = 46, diploid
• 形态学: Lymphoblastoid
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human Burkitt′s lymphoma

细胞系描述

Established in 1963 from Burkitt′s lymphoma in an 11 year old male. Growth is in the form of single cells without attachment and as macroscopically visible clumps containing many hundreds of cells. Resistant to VSV. This cell line carries the latent Epstein-Barr Virus (EBV) genome and is positive for EBNA. RAJI is sometimes referred to as a ′non-producer′; the EBV genome carries deletions attributed to preventing the formation of virus particles. Ethnicity: Black.

应用

Resistance and virus studies: vesicular stomatitis virus (VSV).
RAJI Cell Line human has been used to investigate the transport of V. parahaemolyticus across the Payer′s patch of the intestinal epithelium and it has been co-cultured with Caco-2 (human colorectal adenocarcinoma cell-line) monolayers to study bacterial translocation.

DNA图谱分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 8,11
D5S818: 10,13
D7S820: 10
THO1: 6,7
TPOX: 8,13
vWA: 16,19

培养基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

其他说明

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