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RAJI Cell Line human

85011429

Synonym(s):
GM04671 Cells, P1-Raji Cells

生物来源

human lymph

描述

Human Black, Burkitt′s lymphoma

生长模式

Suspension

核型

2n = 46, diploid

形态学

Lymphoblastoid

产品

Not specified

受体

Not specified

technique(s)

cell culture | mammalian: suitable

相关疾病

cancer

运输

dry ice

储存温度

−196°C

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RAJI Cell Line human 85011429

RAJI Cell Line human

BeWo Cell Line human 86082803

BeWo Cell Line human

RAMOS Cell Line human 85030802

RAMOS Cell Line human

NCI-H322 Cell Line human 95111734

NCI-H322 Cell Line human

biological source

human lymph

biological source

human placenta

biological source

human lymph

biological source

human lung (cervical node metastasis)

description

Human Black, Burkitt′s lymphoma

description

Human choriocarcinoma

description

Human Caucasian Burkitt′s lymphoma

description

Human Caucasian bronchioalveolar carcinoma

growth mode

Suspension

growth mode

Adherent

growth mode

Suspension

growth mode

Adherent

karyotype

2n = 46, diploid

karyotype

modal number =86 range 71-178

karyotype

Modal no. 45

karyotype

Not specified

morphology

Lymphoblastoid

morphology

Epithelial

morphology

Lymphoblast

morphology

Not specified

products

Not specified

products

Gonadotrophin, lactogen and steroid hormones

products

Not specified

products

Not specified

细胞系来源

Human Burkitt′s lymphoma

细胞系描述

Established in 1963 from Burkitt′s lymphoma in an 11 year old male. Growth is in the form of single cells without attachment and as macroscopically visible clumps containing many hundreds of cells. Resistant to VSV. This cell line carries the latent Epstein-Barr Virus (EBV) genome and is positive for EBNA. RAJI is sometimes referred to as a ′non-producer′; the EBV genome carries deletions attributed to preventing the formation of virus particles. Ethnicity: Black.

应用

Resistance and virus studies: vesicular stomatitis virus (VSV).
RAJI Cell Line human has been used to investigate the transport of V. parahaemolyticus across the Payer′s patch of the intestinal epithelium and it has been co-cultured with Caco-2 (human colorectal adenocarcinoma cell-line) monolayers to study bacterial translocation.

DNA图谱分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 8,11
D5S818: 10,13
D7S820: 10
THO1: 6,7
TPOX: 8,13
vWA: 16,19

培养基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

其他说明

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