Merck

NS0 Cell Line from murine myeloma

Mouse myeloma, 85110503

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生物来源

mouse skin

形式

cell suspension

生长模式

Suspension

核型

Not specified

形态学

Lymphoblast

产品

Not specified

受体

Not specified

technique(s)

cell culture | mammalian: suitable

相关疾病

cancer

运输

dry ice

储存温度

−196°C

细胞系来源

Mouse myeloma

细胞系描述

NS0 is a non-Ig secreting, non-light chain-synthesising subclone of NS-1 (P3/NS1/1-Ag4-1 ECACC catalogue no. 85011427). The cells are resistant to 10μM azaguanine, cells die in the presence of HAT medium. The NS0 cell line is the property of the Medical Research Council (MRC). ECACC is unable to supply this cell line to commercial entities unless a license has been first secured from LifeArc (formerly known as MRC Technology). Requests for commercial use of this cell line will be forwarded to LifeArc.

应用

Fusion partner
NS0 Cell Line from murine myeloma has been used to culture NS0 cells expressing CD19scFv (a binding domain of B-lymphocyte antigen CD19) to screen hybridoma clones.

传代培养常规

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells can adhere loosely to the culture vessel surface; remove by tapping against the flask. Cultures that are more than 2 days old may have to be scraped.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免责声明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

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商品

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