Merck

SK.N.SH Cell Line human

from human nerve(bone marrow metastasis; Caucasian neuroblastoma), 86012802

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别名:
NSH Cells, SK-NSH Cells, SKNSH Cells

生物来源

human nerve (bone marrow metastasis; Caucasian neuroblastoma)

生长模式

Adherent

核型

2n = 46, modal no. 47

形态学

Epithelial

产品

Not specified

受体

Not specified

technique(s)

cell culture | mammalian: suitable

相关疾病

metastasis

运输

dry ice

储存温度

−196°C

细胞系来源

Human Caucasian neuroblastoma

细胞系描述

Established from a bone marrow metastasis from a 4-year-old caucasian female suffering with neuroblastoma. The cells have been used as a target cell in cell-mediated cytoxicity tests.

应用

Human SK.N.SH cell line has been used in dark toxicity testing of a chloro-indium-phthalocyanine photosensitizer (In-Pc). It has also been used to study the potential mechanisms and domain requirements of the hHSJ1a (DnaJ heat shock protein family member B2) mediated reduction in superoxide dismutase 1 (SOD1) aggregation.

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 8,13
D5S818: 12
D7S820: 7,10
THO1: 7,10
TPOX: 8,11
vWA: 14,18

培养基

EMEM (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

其他说明

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