MCF7 Cell Line human

86012803, from human breast(adenocarcinoma)

• 别名: IBMF-7 Cells, MCF7 Cells, Michigan Cancer Foundation-7 Cells, ssMCF-7 Cells
• eCl@ss: 32190102

属性

• 生物来源: human breast (adenocarcinoma)
• 生长模式: Adherent
• 核型: 2n = 46, hypertriploid to hypotetraploid
• 形态学: Epithelial-like
• 产品: Not specified
• 受体: Reported to express the oestrogen and progesterone receptor
• technique(s): cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human Caucasian breast adenocarcinoma

细胞系描述

Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of domes. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen (P2 containment). Cells express both the wild type and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.

应用

Tumourigenicity and virus studies: Type B and C viruses

DNA图谱分析

STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids NEAA + 10% Foetal Bovine Serum FBS / FCS.

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C.

其他说明

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