A549 Cell Line human

86012804, from human lung(carcinoma)

• Synonym(s): A-549 Cells, A549 Cells, NCI-A549 Cells

Properties

• 生物来源: human lung (carcinoma)
• 生长模式: Adherent
• 核型: Hypotriploid
• 形态学: Epithelial
• 产品: Lecithin, High amounts of de-saturated fatty acids.
• 受体: Not specified
• technique(s): cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

Description

细胞系来源

Human Caucasian lung carcinoma

细胞系描述

Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.

应用

Enzymatic studies

Human A549 cell line has been used:

• to study the ability of 2-(2,4-dihydroxyphenyl)thieno-1,3-thiazin-4-one (BChTT) to inhibit cancer cell proliferation and identify mechanisms involved in its actions
• to study the effects of insulin and insulin-like growth factor 1 (IGF1) on proliferation and apoptosis in A549 cells
• to culture A549 cells in different media over a long term and identify conditions for promoting alveolar type II differentiation characteristics in A549 cells

DNA图谱分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14

培养基

Ham′s F12K + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% Trypsin or tTrypsin/EDTA; 5% CO₂; 37°C.

其他说明

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