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EMJ human cell line

86021103, IHW Number: 9097

生物来源

human blood

种族

North American Caucasian

性别

female

生长模式

Suspension

核型

Not specified

形态学

Lymphoblastoid

technique(s)

cell culture | mammalian: suitable

运输

dry ice

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一般描述

Homozygous: Yes
Consanguineous: No

细胞系描述

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA图谱分析

A: 0201 0301
Cw: 304
B: 4001
HLA DRA: 102
HLA DRB1: 1302
HLA DRB3: 301
HLA DQAP: 1.4
HLA DQA1: 1021
HLA DQB1: 604
HLA DPA1: 1
HLA DPB1: 0301 0401

血清学分析

HLA A: 2 3
HLA A ief: 2.2 3.2
HLA C: 10
HLA B: 60
HLA B ief: 60
Bw4/bw6: 6
HLA Dw: 19
HLA DR: 13
HLA Dw2: 26
HLA DR52: 52
HLA DQ: 6
HLA DP: 3 4

培养基

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

传代培养常规

Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免责声明

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

Certificate of Analysis

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Certificate of Origin

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