Hep2 (HeLa derivative) Cell Line human

86030501

属性

• 生物来源: human cervix
• 描述: Human Black, cervix carcinoma
• 生长模式: Adherent
• 核型: Modal no. 14
• 形态学: Epithelial
• 产品: Not specified
• 受体: Not specified
• technique(s): cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human cervix carcinoma

细胞系描述

Originated from tumours produced in irradiated-cortisonised weanling rats after injecting with epidermoid carcinoma tissue from the larynx of a 56 year old male. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

应用

Human Hep2 (HeLa derivative) cell line has been used for virus and tumorigenicity studies.

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. After thawing the first subculture may take 7-10 days.

其他说明

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