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Merck

V79

86041102, hamster lung, Fibroblast

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About This Item

分類程式碼代碼:
41106514

product name

V79, 86041102

生物源

hamster lung

增長模式

Adherent

染色體組型

2n=22

形態學

Fibroblast

產品

Not specified

受體

Not specified

技術

cell culture | mammalian: suitable

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Hamster Chinese lung male

細胞系描述

Established after spontaneous transformation of cells isolated from the lung of a normal Chinese hamster (male). The G1 phase in these cells is either absent or very short. Consequently they have been used in studies of the G1 growth period. A clone called V79.4 is available (Sigma Catalogue number. 93010723). V79 is well established in toxicology studies. Stability of karyotype and morphology is making them suitable for gene toxicity assays with low background aberrations. The cells do not express endogenous cytochrome P450 and have been used for the insertion and expression of P450 from human, rat, mouse and fish.

應用

Growth factors and transformation studies, mutagenesis study
V79 has been used to detect structural and numerical chromosome damage by measuring the formation of micronuclei in interphase cells that have been through a mitotic division.

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:12 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

其他說明

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