Hep 3B Cell Line human

from human liver, 86062703

• 别名: HEP3B217 Cells, Hep 3B2.1-7 Cells, Hep3B Cells

属性

• 生物来源: human liver
• 生长模式: Adherent
• 核型: 2n = 46, modal no. 60
• 形态学: Epithelial
• 产品: Alpha-foetoproteins, Hepatitis B Surface Antigen (HBSAg), albumin, transferrin, fibrinogen, complement (C3), alpha-2-macroglobulins
• 受体: Not specified
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系描述

Derived from an 8 year old male. Cells contain integrated Hepatitis B virus genome. However there is currently no evidence that this cell line produces infectious Hepatitis B virus. The cells should be handled under laboratory containment level 2. Ethnicity: Black.

DNA图谱分析

STR-PCR Data:

Amelogenin: X
CSF1PO: 8
D13S317: 12,14
D16S539: 10
D5S818: 13
D7S820: 8,10
THO1: 6,7
TPOX: 9
vWA: 17

培养基

EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

其他说明

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