Merck
All Photos(1)

AtT20

87021902

生物来源

mouse brain

生长模式

Aggregates in suspension

核型

Diploid

形态学

Small round cells growing as large aggregates

产品

Adrenocorticotropic hormone (ACTH)

受体

Not specified

technique(s)

cell culture | mammalian: suitable

相关疾病

cancer

运输

dry ice

储存温度

−196°C

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This Item
CB_94101449CB_93120108CB_94101452
AtT20 87021902

AtT20

AT-1 94101449

AT-1

HTC 93120108

HTC

AT6.1 94101452

AT6.1

biological source

mouse brain

biological source

rat prostate

biological source

rat liver

biological source

rat prostate

growth mode

Aggregates in suspension

growth mode

Adherent

growth mode

Adherent

growth mode

Adherent

karyotype

Diploid

karyotype

Modal no. 62

karyotype

2n = 66, hypertriploid

karyotype

44XY, +4, + 12 del(3)(3q32-36)del(15)p14

morphology

Small round cells growing as large aggregates

morphology

Epithelial

morphology

Epithelial

morphology

Epithelial

products

Adrenocorticotropic hormone (ACTH)

products

Not specified

products

Not specified

products

Not specified

receptors

Not specified

receptors

Not specified

receptors

Dexamethasone phosphate; hydrocortisone

receptors

Not specified

细胞系来源

Mouse LAF1 pituitary gland tumour

细胞系描述

Derived after alternate passage of LAF1 mouse pituitary tumour cells as tumours in animals and in cell culture. Cells produce adrenocorticotropic hormone (ACTH) at approximately the rate of 1000mU/mg of cell protein per week.

应用

Hormone studies

培养基

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS). Cells grow in large aggregates.

传代培养常规

Allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. A subcultivation ratio of 1:2 is recommended. Cells grow in large viable clusters making counting difficult. A seeding densi

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