COLO 205 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87061208, human colon. Round, refractile cells in suspension. Some cuboidal morphology in monolayer.

• 别名: COLO205 Cells
• UNSPSC代码: 41106514

属性

• 生物来源: human colon
• 描述: Human Caucasian colon adenocarcinoma
• 包装: tube of 5 μg 87061208-DNA-5UG; pkg of vial of cells 87061208-1VL
• 生长模式: Suspension
• 核型: Hypertriploid modal no 71-78
• 形态学: Round and refractile cells in suspension. Some cuboidal cells in the monolayer
• 产品: Carcinoembryonic antigen (CEA)
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

一般描述

Isolated from ascitic fluid of a 70 year old Caucasian male with carcinoma of the colon. Produces carcinoembryonic antigen.

细胞系来源

Human Caucasian colon adenocarcinoma

细胞系描述

COLO 205, COLO 201 (Sigma product no. 87091201) and COLO 206 (Sigma product no. 93052620) were all established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). At time of isolation, COLO 205 displayed cuboidal morphology but with increasing passage the depositor reported that COLO 205 tends to display predominantly rounded cells in clumps in suspension with cuboidal cells in the monolayer.

应用

tumourigenic in suitable animals

培养基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Maintain suspension cells at 3-9x10⁵ cells/cm³. Split subconfluent cultures of attached cells 1:3 to 1:6 seeding at 2-3x10⁴ cells/cm²; 5% CO₂; 37°C.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.