Merck

PANC-1 Cell Line human

from human pancreas, 87092802

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别名:
PANC1 Cells, Panc-1-P Cells
eCl@ss:
32190102

生物来源

human pancreas

生长模式

Adherent

核型

2n = 46, hypertriploid

形态学

Epithelial

产品

Not specified

受体

Not specified

technique(s)

cell culture | mammalian: suitable

相关疾病

cancer

运输

dry ice

储存温度

−196°C

细胞系来源

Human Caucasian pancreas

细胞系描述

Established from a pancreatic carcinoma of ductal origin from a 56-year-old Caucasian male. Cells possess the type B phenotype for G6PD. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

应用

Human PANC-1 cell line has been used to study the role of the intermediate filament keratin reorganization on the alignment and migration of cancer cells. It has also been used to study calcium-mediated actin reset (CaAR) in response to physiological changes.

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11
D5S818: 11,13
D7S820: 8,10
THO1: 7,8
TPOX: 8,11
vWA: 15

培养基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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相关内容

Authenticated Pancreatic Cancer Cell Lines for Cancer Research

We offer more than twenty ECACC pancreatic cancer cell lines of human, rat, or mouse origin, including PANC-1. Choose cells based on mutations in KRAS, TP53, SMAD4, and other key genes, or by demonstrated suitability for applications such as toxicity, xenograft models, and drug response studies.

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