Merck

T84 Cell Line human

88021101

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别名:
T-84 Cells

生物来源

human colon (lung metastasis)

描述

Human colon carcinoma

生长模式

Adherent

核型

2n = 46, modal no. 56

形态学

Epithelial

产品

Not specified

受体

Not specified

technique(s)

cell culture | mammalian: suitable

相关疾病

metastasis

运输

dry ice

储存温度

−196°C

细胞系来源

Human colon carcinoma

细胞系描述

Derived from a lung metastasis of colon carcinoma in a 72 year old male. Tumour tissue was inoculated sub-cutaneously and serially transplanted in BALB/c nude mice and subsequently established in in vitro culture. The histological characteristics of the tumour were maintained throughout the transplantation procedure. They have receptors for a range of peptide hormones and neurotransmitters. The cells grow as monolayers, exhibit tight junctions and desmosomes between adjacent cells.

应用

Human T84 cell line has been used for comparative genome analysis of Salmonella enterica serovar Typhimurium definitive type 2 (DT2) isolate 94-213 and S. Typhimurium isolates SL1344, DT104 and D23580. The ability of these isolates to invade culture medium has also been studied. It has also been used to construct and study the suitability of an in vitro model to study chemotherapy-induced mucosal damage.
Tumourigenicity, neurotransmitter and hormone studies

培养基

Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 - 1:4 i.e. seeding at 1-3 x 10,000 cells / cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells grow slowly and may not form a complete monolayer, maintain at high density with a minimum of 25% confluency.

其他说明

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