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product name
Jurkat E6.1 Cell Line human, 88042803, from human blood(leukemic T-cell lymphoblast)
生物源
human blood (leukemic T-cell lymphoblast)
增長模式
Suspension
染色體組型
Pseudodiploid, modal No 46
形態學
Not specified
產品
Interleukin 2 (IL-2), human alpha interferon
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Human leukaemic T cell lymphoblast
細胞系描述
Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages.
應用
IL-2 assay and cloning studies
Jurkat E6.1 cell line has been used to:
- study cell-independent/vector-free delivery of bovine serum albumin-fluorescein conjugate
- determine the cytotoxicity of the cytolethal distending toxin (CDT) holotoxin and its components
- study the expression of bisphenol A exposed estrogen receptor-β (ERβ) and estrogen-related-receptor-α (ERRα) in vitro
DNA分析
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18
培養基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
例行更新培養
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. On resuscitation single cells can be observed, during culture most cells will grow as aggregates.
其他說明
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商品
Application note on how the CellASIC® ONIX2 microfluidic system can be used to analyze caspase-3 mediated apoptosis/cell death and cellular hypoxia in live immune and cancer cell lines.
实验方案
Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.
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