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U-87 MG Cell Line human

from human brain(glioblastoma astrocytoma), 89081402

Synonym(s):
U-87MG Cells, U87 Cells, U87MG Cells

生物来源

human brain (glioblastoma astrocytoma)

生长模式

Adherent

核型

2n = 46

形态学

Epithelial-like

产品

Not specified

受体

Not specified

technique(s)

cell culture | mammalian: suitable

相关疾病

cancer

运输

dry ice

储存温度

−196°C

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CB_85011440CB_86030402CB_09063001
U-87 MG Cell Line human from human brain(glioblastoma astrocytoma), 89081402

U-87 MG Cell Line human

U937 Cell Line human from human lung(lymphoblast), 85011440

U937 Cell Line human

1321N1 Cell Line human from human brain(astrocytoma), 86030402

1321N1 Cell Line human

U-251 MG cell line human 09063001

U-251 MG cell line human

biological source

human brain (glioblastoma astrocytoma)

biological source

human lung (lymphoblast)

biological source

human brain (astrocytoma)

biological source

human brain

growth mode

Adherent

growth mode

Suspension

growth mode

Adherent

growth mode

Adherent

karyotype

2n = 46

karyotype

Not specified

karyotype

Not specified

karyotype

2n = 46

morphology

Epithelial-like

morphology

Lymphoblast

morphology

Glial

morphology

Pleomorphic/astrocytoid

products

Not specified

products

Not specified

products

Not specified

products

Contains GFAP positive cells

receptors

Not specified

receptors

Not specified

receptors

Not specified

receptors

PDGFR alpha and EGFR. See Nistér M. et al., (1988) & (1991)

细胞系来源

Human glioblastoma astrocytoma

细胞系描述

Derived from a malignant glioma from a female patient by explant technique. It is reported to produce a malignant tumour consistent with glioblastoma in nude mice.

应用

Neural studies
U-87 MG cell line has been used to study the effects of the lysosomal destabilizing drug Siramesine on glioblastoma. It has also been used to study calcium-independent disruption of microtubule dynamics by nanosecond pulsed electric fields.

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 8,11
D16S539: 12
D5S818: 11,12
D7S820: 8,9
THO1: 9.3
TPOX: 8
vWA: 15,17

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

其他说明

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