AGS Cell Line human

89090402, human stomach, Not specified

• 分類程式碼代碼: 41106514

属性

• product name: AGS Cell Line human, 89090402
• 生物源: human stomach
• 描述: Human Caucasian gastric adenocarcinoma
• 增長模式: Adherent
• 染色體組型: Hyperdiploid
• 形態學: Not specified
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian gastric adenocarcinoma

細胞系描述

Derived from an adenocarcinoma of the stomach of a 54 year-old Caucasian female with no prior anti-cancer treatment. AGS cells are persistently infected with parainfluenza virus type 5 (PIV5).

應用

AGS human cell line has been used:

• to study the role of obestatin/G protein-coupled receptor 39 (GPR39) in gastric cancer progression
• to determine expression levels of colon cancer associated transcript -1 (CCAT1)
• to study the adhesion of Lactobacillus sps. to the gastric mucosa

Anti-tumour testing

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 11,13
D5S818: 9,12
D7S820: 10,11
THO1: 6,7
TPOX: 11,12
vWA: 16,17

培養基

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 0.05% EDTA; 5% CO₂; 37°C.

其他說明

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