Merck

LNCap Clone FGC Cell Line human

89110211, from human prostate(carcinoma)

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别名:
LNCaP-FGC Cells

生物来源

human prostate (carcinoma)

生长模式

Adherent

核型

Pseudodiploid male; seven marker chromosomes, modal number 46, range 33 to 91

形态学

Epithelial-like

产品

Prostate acid phosphatase, prostate specific antigen

受体

Androgen, estrogen

technique(s)

cell culture | mammalian: suitable

相关疾病

metastasis

运输

dry ice

储存温度

−196°C

细胞系来源

Human Caucasian prostate carcinoma

细胞系描述

Derived from a metastasis at the left supraclavicular lymph node of a 50 year old patient with a confirmed diagnosis of metastatic prostate carcinoma. Growth and acid phosphatase production is affected by 5-alpha-dihydrotestosterone. They do not form a uniform monolayer and attach only lightly to the substrate. When shipped, cells detach from flask and can either be incubated 24-48 hours to allow attachment or be collected by centrifugation (150xg, 15 minutes) and reseeded.

应用

LNCap Clone FGC Cell Line has been used:
  • to study the effect of sex steroid hormones in regulating the expression of oligoadenylate synthetase 1 (OAS1)
  • to study the role of curcumin and resveratrol in redox control and ability to promote apoptosis
  • to investigate low molecular weight thiols present in the deproteinized extract of the cell line after derivatization

培养基

RPMI 1640 + 2mM Glutamine + 1.0 mM sodium pyruvate + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.i.e. seeding at 1-3 x 10,000 cells/cm2 Cells grow slowly in clusters and can be disaggregated by repeated pipetting. After subculture cells may take up to 48 hours to reattach. Do not disturb during this period. Medium is rapidly acidified, therefore change twice per week.

其他说明

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