Merck

PC-9 (formerly known as PC-14)

90071810

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别名:
PC-9/S1 Cells, PC-9S1 Cells, PC9 Cells

生物来源

human lung

生长模式

Adherent/suspension

核型

Not specified

形态学

Heterogeneous mixture of round cells and spindle shaped cells

产品

Not specified

受体

Not specified

technique(s)

cell culture | mammalian: suitable

相关疾病

cancer

运输

dry ice

储存温度

−196°C

细胞系来源

Human adenocarcinoma

细胞系描述

Derived from a human adenocarcinoma from lung tissue which remains undifferentiated.

PC-14 was originally deposited with the Riken BioResource Center as a cell line derived from human lung adenocarcinoma (undifferentiated type) in 1989. The RIKEN BioResource Center subsequently informed the European Collection of Cell Cultures (ECACC) that short tandem repeat (STR) DNA profile analysis, undertaken in collaboration Immuno-Biological Laboratories Co., Ltd Japan, has shown this cell line to be identical to PC-9 a cell line derived from human lung adenocarcinoma (differentiated type). The misidentification occurred prior to the cell line being deposited at the RIKEN BioResource Center. The name of the cell line has been changed to PC-9 to reflect this finding.

应用

PC-9 cell line has been used to study the effects of α-tocopherol addition on various cytotoxic drugs.
Tumourigenicity studies

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 8
D16S539: 9
D5S818: 11
D7S820: 10
THO1: 7
TPOX: 11
vWA: 17

培养基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Seed cells at 2-4 x 10,000 cells/cm2; 5% CO2; 37°C. Remove attached cells by brief treatment with 0.25% trypsin or trypsin/EDTA.  

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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ECACC Nucleic Acids

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

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