MC116

90110501

属性

• 生物来源: human lymph
• 生长模式: Suspension
• 核型: Not specified
• 形态学: Lymphoblast
• 产品: Surface Immunoglobulin M (IgM), pentameric Immunoglobulin M (IgM)
• 受体: Not specified
• technique(s): cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human lymphoma

细胞系描述

Derived from the pleural effusion of a patient with undifferentiated lymphoma. The cells are EBNA negative, express surface IgM Lambda and secrete pentameric IgM which does not bind to protein A. Complement and Fc receptors were not detected.

应用

Hormone and tumourigenicity studies

DNA图谱分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 12
D5S818: 12
D7S820: 10,13
THO1: 9.3,10
TPOX: 8,10
vWA: 15,17

培养基

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

传代培养常规

Maintain cultures between 100,000 to 1,000,000 cells/ml; 5% CO₂; 37°C. A maximum cell density of 2-4x1,000,000 cells/ml is achieved.

其他说明

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