PC-3 Cell Line human

Caucasian prostate adenocarcinoma, 90112714

• 别名: PC.3 Cells, PC3 Cells
• eCl@ss: 32190102

属性

• 生物来源: human prostate
• 生长模式: Adherent
• 核型: 2n = 46, triploid, modal no. 62
• 形态学: Epithelial
• 产品: Not specified
• 受体: Not specified
• technique(s): cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human Caucasian prostate adenocarcinoma

细胞系描述

Established from a grade 4 prostatic adenocarcinoma from a 62 year old male Caucasian. The cells grow in agar and produce tumours in nude mice. Exhibit low acid phosphatase and testosterone-5-α reductase activity. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

应用

PC-3 cell line has been used to study the antiproliferative effects of F. mughlea Pesmen on cancer cells. It has also been used to study the effects of palmitoylcarnitine (palcar) on the gene expression and pro-inflammatory pathways in prostate cancer.

Tumourigenicity studies

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 11
D5S818: 13
D7S820: 8,11
THO1: 6,7
TPOX: 8,9
vWA: 17

培养基

Coons Modified Ham’s F12 + 2mM Glutamine + 7% Foetal Bovine Serum (FBS).OR Kaign’s modified Ham’s F12 + 45mg/L ascorbic acid + 18 mg/L Inositol + 2mM Glutamine + 7% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Medium change every 5 days. The initial subculture interval after cells are thawed may be longer than 7-9 days.

其他说明

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