J774A.1 Cell Line from mouse

91051511

• 别名: J774 A1 Cells, J774.A1 Cells, J774A1 Cells

属性

• 生物来源: mouse blood
• 描述: Mouse BALB/c monocyte macrophage
• 生长模式: Semi-adherent
• 核型: Not specified
• 形态学: Not specified
• 产品: Interleukin 1 (IL-1), lysozyme
• 受体: Immunoglobulin, complement
• technique(s): cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Mouse BALB/c monocyte macrophage

细胞系描述

Derived from a tumour in a female BALB/c mouse. Cell line produces IL-5 and lysozyme.

应用

J774A.1 cell line has been used to test the cytotoxicity of [1,2,3]triazolo[1,5-a]pyridinium salts (having leishmanicidal activity) from triazolopyridines. It has also been used to determine the antitrypanosomatid activity of flavonoid glycosides isolated from Delphinium sps.

Metabolic studies

培养基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Cells are subcultured by harvesting attached and suspension cells. Remove majority of medium and centrifuge to harvest cells growing in suspension. Scrape adherent cells remaining in the flask. Resuspend cell pellet from centrifugation and combine with scraped cells. Seed into new flask at 1:3 to 1:4 split ratio i.e. seeding at 1-3 x 10,000 cells/cm² Incubate at 37°C; 5% CO₂. Please Note :- On receipt growing order cells may be in suspension. The cells and media should be removed from the flask and spun down to form a pellet. This should then be re-suspended in fresh media and a cell count performed. The cells should then be reseeded at 2-4 x 10E4/cm and should begin to show signs of re-attachment within 48 hours.

其他说明

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