Merck
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RAW 264.7 Cell Line murine

91062702, Macrophage from blood

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Synonym(s):
RAW264 7 Cells, RAW264.7 Cells

生物来源

mouse blood

生长模式

Semi-adherent

核型

Not specified

形态学

Macrophage

产品

Lysozyme

受体

Immunoglobulin, complement

technique(s)

cell culture | mammalian: suitable

相关疾病

cancer

运输

dry ice

储存温度

−196°C

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RAW 264.7 Cell Line murine 91062702, Macrophage from blood

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EL4 Cell Line murine Lymphoblast from blood, 85023105

EL4 Cell Line murine

biological source

mouse blood

biological source

mouse skin (fibroblast)

biological source

human blood

biological source

mouse blood

growth mode

Semi-adherent

growth mode

Adherent

growth mode

Suspension

growth mode

Suspension

karyotype

Not specified

karyotype

Not specified

karyotype

Not specified

karyotype

2n = 39

morphology

Macrophage

morphology

Fibroblast

morphology

Lymphoblastoid

morphology

Lymphoblast

products

Lysozyme

products

Not specified

products

Not specified

products

A surface antigen induced by leukaemia type G virus; H-2b and Thy-1.2 antigens; Interleukin 4 (IL-4), H-2b, Thy1.2

receptors

Immunoglobulin, complement

receptors

Not specified

receptors

Immunoglobulin E (IgE) binding factor, Calcitonin

receptors

Not specified

细胞系来源

Mouse monocyte macrophage

细胞系描述

Established from an ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS.

应用

Metabolic studies
RAW 264.7 cell line has been used to study the anti-inflammatory effects of pristimerin. It has also been used to test the internalization and intracellular survival of Listeria monocytogenes.

培养基

DMEM + 2mM Glutamine + 10% FBS / FCS (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm2; 5% CO2; 37°C. Remove the cells mechanically. Cells are semi-adherent, i.e. some cells grow in suspension, some loosely attach to the surface and others flattened out and attached to the flask. Cells should not be allowed to overgrow and become confluent as this can lead to loss of the flattened adherent cell characteristic.

其他说明

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