HT29 Cell Line human

Caucasian colon adenocarcinoma grade II, 91072201

• Synonym(s): HT29 Cells

Properties

• 生物来源: human colon
• 生长模式: Adherent
• 核型: 2n = 46, hypertriploid
• 形态学: Epithelial
• 产品: Secretory component of Immunoglobulin A (IgA), Carcinoembryonic antigen (CEA)
• 受体: Not specified
• technique(s): cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

Description

细胞系来源

Human Caucasian colon adenocarcinoma

细胞系描述

Isolated from a primary tumour in a 44 year old Caucasian female. Forms a well-differentiated adenocarcinoma consistent with colony primary, grade I. Tumours also form in steroid treated hamsters. Has the following HLA profile A1,3; B12,17; Cw5.

应用

HT29 Cell Line has been used to determine viral titres of human parechovirus. It has also been used to study the ability of Bifidobacterium and Lactobacillus strains to counteract the toxic effect of C. difficile LMG21717.

Tumourigenicity studies

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 8,9
vWA: 17,19

培养基

McCoy′s 5A + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% Trypsin; 5% CO₂; 37°C.

其他说明

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