HCT 116 Cell Line human

colon carcinoma, 91091005

• Synonym(s): HCT116 Cells

Properties

• 生物来源: human colon
• 生长模式: Adherent
• 核型: Modal no. 45
• 形态学: Epithelial-like
• 产品: Not specified
• 受体: Not specified
• technique(s): cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

Description

细胞系来源

Human colon carcinoma

细胞系描述

One of 3 strains of malignant cells isolated from a male with colonic carcinoma. Cells are tumourigenic in nude mice and form colonies on agarose. Growth and plating efficiency are enhanced by using a feeder layer of murine fibroblasts.

应用

HCT 116 Cell Line has been used to:

• study the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA)
• study the effects of different amounts of transferrin within the polyethyleneimine (PEI)-small interfering RNA (siRNA) complexes on the uptake of fluorescently-labelled siRNA
• determine the effect of PTS (polyamine transport system) in the selective uptake of Quilamine HQ1-44, an iron chelator

Tumourigenicity studies

DNA图谱分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7,10
D13S317: 10,12
D16S539: 11,13
D5S818: 10,11
D7S820: 11,12
THO1: 8,9
TPOX: 8
vWA: 17,22

培养基

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他说明

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