Merck

MDA-MB-231 Cell Line human

92020424, human breast adenocarcinoma

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别名:
MD Anderson-Metastatic Breast-231 Cells, MDA-MB231 Cells, MDA231-BRE Cells, MDAMB231 Cells
eCl@ss:
32190102

生物来源

human breast

生长模式

Adherent

核型

Modal no.′s 62 and 64, near triploid

形态学

Epithelial

产品

Not specified

受体

Not specified

technique(s)

cell culture | mammalian: suitable

相关疾病

cancer

运输

dry ice

储存温度

−196°C

细胞系来源

Human Caucasian breast adenocarcinoma

细胞系描述

Isolated from pleural effusions of a breast cancer patient.

应用

MDA-MB-231 has been used to study:
  • the changes in the morphological pattern and gene expression of cells when grown in the presence of sera positive for anti-carbonic anhydrase I (CA I) autoantibodies
  • the effects of normothermic conditioned microwave irradiation on cultured cells using an irradiation system
  • the transendothelial migration rates of the cells under different shear stress levels

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 12,13
D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 8,9
THO1: 7,9.3
TPOX: 8,9
vWA: 15,18

培养基

L15+ 2mM Glutamine + 15% Foetal Bovine SerumFBS / FCS.

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e. seeding at 3-6x10,000 cells/cm2 using 0.05% Trypsin or Trypsin/EDTA; 37°C. CO2 is not required.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免责声明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

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ECACC Nucleic Acids

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

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