U-2 OS Cell Line human
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92022711, human bone, Not specified
别名:
U-2OS Cells, U2-OS Cells, U20S Cells, U2OS Cells
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About This Item
UNSPSC代码:
41106514
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生物来源
human bone
包装
tube of 5 μg 92022711-DNA-5UG
pkg of vial of cells 92022711-1VL
生长模式
Adherent
核型
Not specified
形态学
Not specified
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
细胞系来源
Human Osteosarcoma
细胞系描述
Cell line derived in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.
应用
U-2 OS has been used to study:
- the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA)
- the interaction of human single-stranded DNA binding protein 1 (hSSB1) with bloom syndrome protein helicase (BLM helicase)
- calcium-mediated actin reset (CaAR) in response to physiological changes
DNA图谱分析
STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 13
D16S539: 11,12
D5S818: 11
D7S820: 11,12
THO1: 6,9.3
TPOX: 11,12
vWA: 14,18
CSF1PO: 13
D13S317: 13
D16S539: 11,12
D5S818: 11
D7S820: 11,12
THO1: 6,9.3
TPOX: 11,12
vWA: 14,18
培养基
McCoy′s 5a medium with 1.5 mM Glutamine; 10% Foetal Bovine Serum (FBS).
传代培养常规
Split subconfluent 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new culture flasks.
其他说明
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商品
Cell cycle regulates vital processes like DNA repair, cancer prevention. Four stages: G1, S, G2, M. NTPs don't permeate membranes.
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