Merck
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U-2 OS Cell Line human

92022711, osteosarcoma

All Photos(1)
Synonym(s):
U-2OS Cells, U2-OS Cells, U20S Cells, U2OS Cells

生物来源

human bone

生长模式

Adherent

核型

Not specified

形态学

Not specified

产品

Not specified

受体

Not specified

technique(s)

cell culture | mammalian: suitable

相关疾病

cancer

运输

dry ice

储存温度

−196°C

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CB_89050205CB_12021502CB_12021503
U-2 OS Cell Line human 92022711, osteosarcoma

U-2 OS Cell Line human

SAOS-2 Cell Line human 89050205, primary osteogenic sarcoma

SAOS-2 Cell Line human

U2A cell line human 12021502

U2A cell line human

U3A Cell line human 12021503

U3A Cell line human

biological source

human bone

biological source

human bone

biological source

human fibrosarcoma

biological source

human fibrosarcoma

growth mode

Adherent

growth mode

Adherent

growth mode

Adherent

growth mode

-

karyotype

Not specified

karyotype

2n = 46, (P1) Hyperploid to hypopentaploid

karyotype

-

karyotype

-

morphology

Not specified

morphology

Epithelial-like

morphology

Epithelial

morphology

Epithelial

products

Not specified

products

Not specified

products

-

products

-

receptors

Not specified

receptors

Not specified

receptors

-

receptors

-

细胞系来源

Human Osteosarcoma

细胞系描述

Cell line derived in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.

应用

U-2 OS has been used to study:
  • the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA)
  • the interaction of human single-stranded DNA binding protein 1 (hSSB1) with bloom syndrome protein helicase (BLM helicase)
  • calcium-mediated actin reset (CaAR) in response to physiological changes

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 13
D16S539: 11,12
D5S818: 11
D7S820: 11,12
THO1: 6,9.3
TPOX: 11,12
vWA: 14,18

培养基

McCoy′s 5a medium with 1.5 mM Glutamine; 10% Foetal Bovine Serum (FBS).

传代培养常规

Split subconfluent 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new culture flasks.

其他说明

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