HeLa Cell Line human

93021013, epitheloid cervix carcinoma

• Synonym(s): HeLa Cells, HeLa-CCL2 Cells, Henrietta Lacks Cells

Properties

• 生物来源: human cervix
• 生长模式: Adherent
• 核型: aneuploidy
• 形态学: Epithelial
• 产品: Not specified
• 受体: Not specified
• technique(s): cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

Description

细胞系来源

Human cervix epitheloid carcinoma

细胞系描述

Derived from a cervical carcinoma from a 31 year old female. This was the first aneuploid line derived from human tissue maintained in continuous cell culture. Susceptible to Poliovirus type I and adenovirus type 3. Identified as a contaminant in many other cell lines. The cells should be handled under laboratory containment level 2. Ethnicity: Black.

应用

HeLa cell line has been used to study whether Zika virus (ZIKV) infection alters centrosome number and spindle positioning in neural progenitor cells. It has also been used to evaluate the cytotoxicity of extracts, fruits and jams from Sorbus sps.

Virus studies

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 12,14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino AcidsNEAA + 10% Foetal Bovine Serum FBS / FCS.

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1.3x10,000 cells/cm² using 0.25% Trypsin/EDTA; 5% CO₂; 37°C.

其他说明

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