NIH 3T3 Cell Line murine

93061524

• 别名: 3T3 Cells, Mouse 3T3 Cells, NIH/3T3 Cells, NIH3T3 Cells, Swiss 3T3 Cells, Swiss3T3 Cells

属性

• 生物来源: mouse embryo
• 描述: Embryonic fibroblast
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Fibroblast
• 产品: Not specified
• 受体: Not specified
• technique(s): cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Mouse Swiss NIH embryo

细胞系描述

Established from a NIH Swiss mouse embryo. These cells are sensitive to sarcoma virus focus formation and leukemia virus propogation. Cells have now lost their contact inhibition.

应用

DNA transfection studies

NIH 3T3 Cell Line has been used to:

• study calcium-mediated actin reset (CaAR) in response to physiological changes
• study the effects of activation of the transient receptor potential vanilloid 3 channel (TRPV3) on adipocyte differentiation
• determine the viral titer of the retroviral construct -ST40hTNFα

培养基

DMEM + 2mM Glutamine + 10% Calf Serum (CS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Do not allow culture to become fully confluent; the use of fetal calf serum is not recommended.

其他说明

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