生物源
human pancreas
描述
Human primary pancreatic adenocarcinoma
包裝
tube of 5 μg 93120816-DNA-5UG
pkg of vial of cells 93120816-1VL
增長模式
Adherent
染色體組型
2n = 59, near triploid
形態學
Epithelial
產品
Mucin
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Human primary pancreatic adenocarcinoma
細胞系描述
Derived from a 61 year old female with a primary adenocarcinoma of the pancreas. BxPC-3 cells produce mucin and the tumour produced in a nude mouse is moderately well to poorly differentiated like the primary adenocarcinoma.
應用
BXPC3 cell line has been used to determine the biological and therapeutic importance of gastrin gene expression in pancreatic adenocarcinomas.
Tumourigenicity studies
DNA分析
STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 11
D16S539: 9,11
D5S818: 11
D7S820: 10,13
THO1: 9
TPOX: 8
vWA: 14,18
CSF1PO: 13
D13S317: 11
D16S539: 9,11
D5S818: 11
D7S820: 10,13
THO1: 9
TPOX: 8
vWA: 14,18
培養基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
其他說明
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