LS 123

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94120801, human colon, Epithelial

• UNSPSC代码: 41106514

属性

• 生物来源: human colon
• 包装: tube of 5 μg 94120801-DNA-5UG; pkg of vial of cells 94120801-1VL
• 生长模式: Adherent
• 核型: Modal no. 63 (2n=46), hypotriploid
• 形态学: Epithelial
• 产品: Carcinoembryonic antigen (CEA)
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human colon adenocarcinoma

细胞系描述

Established in 1974 from the second in a series of 3 primary colonic tumours resected from a 65 year old Caucasian female, which was classified as a Duke′s B mucin producing colon adenocarcinoma. LS 123 is aneuploid, fails to grow progressively in nude mice and forms colonies only in enriched semisolid medium developed for tumour stem cells. The cells will grow in either low serum or serum free medium. In chick embryonic skin assays the cells grow as a well differentiated abnormal colonic epithelium with little mitotic activity and low invasiveness. LS 123 exhibits several properties associated with neoplasia, but have little invasive potential and may represent an early stage in tumour development.

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 9,13
D16S539: 13
D5S818: 11
D7S820: 9
THO1: 7,8
TPOX: 9
vWA: 16

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (Heat inactivated).

传代培养常规

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-2x10,000 cells/cm² using trypsin 0.25% or trypsin/EDTA; 5% CO₂, 37°C.

其他说明

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