Merck

DMS 153

95062827

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生物来源

human lung (liver metastasis)

生长模式

Semi-adherent aggregates

核型

Modal no. 41-42

形态学

Not specified

产品

Not specified

受体

Bombesin, Epidermal growth factor (EGF)

technique(s)

cell culture | mammalian: suitable

相关疾病

metastasis

运输

dry ice

储存温度

−196°C

细胞系来源

Human Caucasian lung small cell carcinoma, metastasis to liver

细胞系描述

DMS 153 was isolated from the liver of a 44 year-old male patient at autopsy who had been treated with cytoxan and methotrexate. The cells were identified as small cell carcinoma of the lung (SCLC) and secrete moderate quantities of calcitonin (CT) and calcitonin gene related peptide (CGRP). Production of adrenocorticotropin (ACTH), bombesin (BN), 17 β-estradiol and oxytocin-neurophysin (OT-NP) has also been reported. The expression of cell surface antigens Leu-7, HLA-DR, My 23 (defined by AML-2-23 monoclonal antibody), the AML-1-99 defined antigen and CR3 receptor (defined by the OKM antibody) has been shown. DMS 153 cells produce receptors for BN and epidermal growth factor (EGF) and are tumourigenic in athymic, nude mice.

应用

Human lung tumour model

DNA图谱分析

STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 10
D13S317: 13
D16S539: 12,13
D5S818: 12
D7S820: 8,10
THO1: 7,9.3
TPOX: 11
vWA: 14,15

培养基

Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Cells grow in large clumps.

其他说明

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