Ishikawa Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 99040201, human uterus, Epithelial-like

• 别名: ISHI Cells, Ishikawa Cells
• UNSPSC Code: 41106514
• Biological source: human uterus
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Epithelial-like

属性

• biological source: human uterus
• packaging: tube of 5 μg 99040201-DNA-5UG, pkg of vial of cells 99040201-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial-like
• products: Not specified
• receptors: Oestrogen, progesterone
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

说明

Application

Ishikawa Cell Line human has been used to study the effect of 1,25-Dihydroxy-Vitamin D3 (1,25(OH)2D3) on cytosolic pH regulation and glycolytic flux. It has also been used to study endometrial adenocarcinoma.

Biochem/physiol Actions

Human Asian endometrial adenocarcinoma

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 9,12
D16S539: 9
D5S818: 10,11
D7S820: 9,10
THO1: 9,10
TPOX: 8
vWA: 14,17

The cell line Ishikawa was established from an endometrial adenocarcinoma from a 39 year old woman. The cells induced well differentiated adenocarcinoma in athymic nude mice. Estrogen and progesterone receptors were demonstrated both in cell culture and in induced tumours. Ishikawa has been reported to produce corticotropin-releasing hormone, placental alkaline phosphatase and chorionic gonadotrophin, and responded to steroid hormones. The cell line has been eradicated from mycoplasma at ECACC using BM-cyclin. Ishikawa was temporarily removed from the web site whilst an authentication issue was investigated. Following collaboration with key investigators in this field, ECACC is confident that this cell line is Ishikawa.

Preparation Note

MEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37ºC; cells tend to pile up.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].