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CHY5S

Sigma-Aldrich

α-糜蛋白酶 来源于牛胰腺

≥40 units/mg protein, vial of 5 mg

CAS号:
EC 号:
MDL编号:
NACRES:
NA.54

质量水平

200

描述

aseptically filled

类型

Type IV-S

形式

solid

specific activity

≥40 units/mg protein

分子量

25 kDa

组成

protein, ≥85% UV

包装

vial of 5 mg

UniProt登记号

储存温度

−20°C

Gene Information

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应用

The enzyme from Sigma has been used to assess the effect of limited proteolysis with α-chymotrypsin on the sperm penetration.
α-Chymotrypsin from bovine has been used in a study to inform proteasome inhibition in order to advance anticancer research. α-Chymotrypsin from bovine has also been used in a study that functionalized surface anchored poly(methylhydrosiloxane) thin films on oxidized silicon wafers.
α-Chymotrypsin from bovine pancreas has been used:
  • as a supplement for the collection of semen into Tris diluent
  • as one of the proteases in the analysis of major histocompatibility complex (MHC) class II protease sensitivity assay
  • as a component in YEPD broth for biofilm dispersion assay
  • in the preparation of chitinase–chymotrypsin–DMSO buffer (CCD buffer) for enzymatic digestion of larvae

生化/生理作用

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. Its pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu2+ and Hg2+.
一种丝氨酸蛋白酶,能将与芳香烃或其他大型疏水性侧链(Tyr、Trp、Phe、Met、Leu)结合的肽从键的羧基端水解。

单位定义

在 pH 7.8 和 25°C 下,一单位本品每分钟可水解 1.0μmol BTEE。

分析说明

由 A1%/280 测定的蛋白质

警示用语:

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 1

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

分析证书

原产地证书 (CofO)

A triad of molecular regions contribute to the formation of two distinct MHC class II conformers
Drake L A and Drake J R
Molecular Immunology, 74, 59-70 (2016)
Effect of enzyme treatment and mechanical removal of alpaca (Vicugna pacos) seminal plasma on sperm functional integrity
Morton K M, et al.
JOURNAL-SHANDONG UNIVERSITY OF SCIENCE AND TECHNOLOGY NATURAL SCIENCE, 5, 62-81 (2012)
MrkD (1P) from Klebsiella pneumoniae IA565 allows for co-existence with Pseudomonas aeruginosa and protection from protease-mediated biofilm detachment
Childers B M, et al.
Infection and Immunity, 74, IAI-00521 (2013)
Immunofluorescent antibody staining of intact Drosophila larvae
Manning L and Doe C Q
Nature Protocols, 12(1), 1-1 (2017)
K L Honea et al.
Journal of assisted reproduction and genetics, 10(4), 255-260 (1993-05-01)
This study was designed to assess the impact of limited proteolysis with alpha-chymotrypsin on the sperm penetration assay (SPA) of infertile patients and to identify a group whose results would normalize with this pretreatment. Further, the application of this treatment

实验方案

Procedure for Enzymatic Assay of α-Chymotrypsin (EC 3.4.21.1)

Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.

相关内容

α-胰凝乳蛋白酶的酶学测定程序(EC 3.4.21.1)

按照我们的程序进行胰凝乳蛋白酶活性测定。该α-胰凝乳蛋白酶测定将会指导您完成整个过程以及必要的计算。

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