Merck

CLL1033

Sigma-Aldrich

U2OS GFP-LMNB1

登录查看公司和协议定价

NACRES:
NA.81

生物来源

human female bone (Source Disease: Osteosarcoma)

质量水平

OMIM登记号

储存温度

−196°C

基因信息

human ... lamin B1(4001)

一般描述

LMNB1 (lamin B1) is a B type lamin protein. LMNB1 is abundantly expressed in various cell types. This gene is located on human chromosome 5q23. The nuclear lamina, an intermediate filament meshwork, is made of LmnB1, LmnB2, A type (LmnA/C) and associated inner nuclear membrane proteins.
This product was derived from ATCC Cat. No. HTB-96.
U2OS GFP-LMNB1 are osteosarcoma, epithelial cells, from a human caucasian female (aged 15 years), with a GFP-tagged modification.

应用

This product is a human U2OS cell line in which the genomic LaminB1 gene has been endogenously tagged with a Green Fluorescent Protein (GFP) gene using CompoZr® Zinc Finger Nuclease technology. Integration resulted in endogenous expression of the fusion protein in which GFP is attached to the N-terminus of LaminB1. Fluorescence imaging shows the characteristic LaminB1 nuclear expression. This stable cell line was expanded from a single clone. The target′s gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.
To learn more, please visit the Cellular Reporter Cell Line webpage

生化/生理作用

Lamin B1 (LMNB1) is essential for proper chromosome condensation in interphase nuclei. It also participates in the distribution of heterochromatin and modulation of gene expression and splicing. Mutations in LMNB1 (lamin B1) gene results in the adult onset demyelinating disease autosomal dominant leukodystrophy (ADLD).
Nuclear lamin B1 encoded by LMNB1 gene is an intermediate filament protein of the nuclear envelope. The protein has transcriptional co-regulatory activity. It plays a key role in DNA replication, cellular aging and stress responses. Overexpression of the gene has been observed in hepatocellular carcinoma (HCC) patients. Thus, LMNB1 can be used as a potential biomarker for HCC.

特点和优势

GFP-tagged on chromosome 5q23.2
The U2OS cells are adherent, with a doubling time of approx. 29 hours.

组分

U2OS GFP-LMNB1 cells

质量

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

制备说明

Media Renewal changes two to three times per week.

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1:3-1:6

The base medium for this cell line is McCoy′s 5A1 Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration of 10%.

The cell freezing medium is DMSO 1X (Cat. No. C6164).

法律信息

CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

为方便起见,与您过往购买产品相关的文件已保存在文档库中。

访问文档库

难以找到您所需的产品或批次号码?

在网站页面上,产品编号会附带包装尺寸/数量一起显示(例如:T1503-25G)。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).

示例

T1503
货号
-
25G
包装规格/数量

其它示例:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

遇到问题?欢迎随时联系我们技术服务 寻求帮助

批号可以在产品标签上"批“ (Lot或Batch)字后面找到。

Aldrich 产品

  • 如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。

  • 如果您查询到的批号含有填充代码(例如05427ES-021),请输入去除填充代码-021的批号:05427ES。

  • 如果您查询到的批号含有填充代码(例如 STBB0728K9),请输入去除填充代码K9的批号:STBB0728。

未找到您寻找的产品?

部分情况下,可能未在线提供COA。如果搜索不到COA,可在线索取。

索取COA

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门