- CLL1035
All Photos(2)
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生物来源
human female bone (Source Disease: Osteosarcoma)
质量水平
OMIM登记号
储存温度
−196°C
Gene Information
human ... beta actin(60)
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This Item | CLL1032 | CLL1033 | 577952 |
|---|---|---|---|
| biological source human female bone (Source Disease: Osteosarcoma) | biological source human female bone (Source Disease: Osteosarcoma) | biological source human female bone (Source Disease: Osteosarcoma) | biological source - |
| OMIM accession no. | OMIM accession no. | OMIM accession no. | OMIM accession no. - |
| storage temp. −196°C | storage temp. −196°C | storage temp. −196°C | storage temp. - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
| Gene Information human ... beta actin(60) | Gene Information human ... beta actin(60) | Gene Information human ... lamin B1(4001) | Gene Information - |
一般描述
U2OS RFP-ACTB are osteosarcoma, epithelial cells, from a human caucasian female (aged 15 years), with a RFP-tagged modification.
应用
This product is a human U2OS cell line in which the genomic ACTB gene has been endogenously tagged with a Red Fluorescent Protein (RFP) gene using CompoZr® Zinc Finger Nuclease technology. Integration resulted in endogenous expression of the fusion protein in which RFP is attached to the N-terminus of actin. Fluorescence imaging shows that the actin can polymerize to form the characteristic pattern of actin filaments. Addition of Cytochalasin B to the cell line causes the expected shortening of actin filaments. This stable cell line was expanded from a single clone. The target′s gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.
To learn more, please visit the Cellular Reporter Cell Line webpage
特点和优势
RFP-tagged on chromosome 7p22.1
The U2OS cells are adherent, with a doubling time of approx. 29 hours.
组分
U2OS RFP-ACTB cells
质量
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.
制备说明
Media Renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3-1:6
The base medium for this cell line is McCoy′s 5A1 Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration of 10%.
The cell freezing medium is DMSO 1X (Cat. No. C6164).
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3-1:6
The base medium for this cell line is McCoy′s 5A1 Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration of 10%.
The cell freezing medium is DMSO 1X (Cat. No. C6164).
法律信息
CompoZr is a registered trademark of Sigma-Aldrich Co. LLC
Certificate of Analysis
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Certificate of Origin
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Data Sheets
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