Merck

CLL1036

Sigma-Aldrich

U2OS GFP-HMGA1

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生物来源

human female bone (Source Disease: Osteosarcoma)

质量水平

OMIM登记号

储存温度

−196°C

基因信息

human ... HMGA1(3159)

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此商品
CLL1037CLL1038CLL1136
U2OS GFP-HMGA1

CLL1036

U2OS GFP-HMGA1

U2OS GFP-ACTB RFP-TUBA1B

CLL1037

U2OS GFP-ACTB RFP-TUBA1B

U2OS BFP-LMNB1 RFP-ACTB

CLL1038

U2OS BFP-LMNB1 RFP-ACTB

U2OS细胞GFP-NUP98

CLL1136

U2OS细胞GFP-NUP98

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

OMIM accession no.

600701

OMIM accession no.

102630, 602530

OMIM accession no.

102630, 150340

OMIM accession no.

601021

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Gene Information

human ... HMGA1(3159)

Gene Information

human ... ACTB(60), TUBA1B(10376)

Gene Information

human ... ACTB(60), LMNB1(4001)

Gene Information

human ... NUP98(4928)

一般描述

This product was derived from ATCC Cat. No. HTB-96.

U2OS GFP-HMGA1 are osteosarcoma, epithelial cells, from a human caucasian female (aged 15 years), with a GFP-tagged modification.

应用

This product is a human U2OS cell line in which the genomic HMGA1 gene has been endogenously tagged with a Green Fluorescent Protein (GFP) gene using CompoZr® Zinc Finger Nuclease technology. Integration resulted in endogenous expression of the fusion protein in which GFP is attached to the C-terminus of HMGA1. Fluorescence imaging shows characteristic HMGA1 nuclear expression. This stable cell line was expanded from a single clone. The target′s gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.
To learn more, please visit the Cellular Reporter Cell Line webpage

特点和优势

GFP-tagged on chromosome 6p21.

The U2OS cells are adherent, with a doubling time of approx. 29 hours.

组分

U2OS GFP-HMGA1 cells

质量

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

制备说明

Media Renewal changes two to three times per week.

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1:3-1:6

The base medium for this cell line is McCoy′s 5A1 Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration of 10%.

The cell freezing medium is DMSO 1X (Cat. No. C6164).

法律信息

CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

相关产品

产品编号
说明
价格

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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