CLL1222

Safe Harbor Landing Pad Cell Line HCT-116 Cancer Cells

Properties

• 生物来源: human male colorectal tissue (Source Disease: Colorectal carcinoma)
• 质量水平: 200
• 形式: frozen liquid (Vial of Frozen Cells)
• 生长模式: Adherent
• technique(s): cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

Description

一般描述

The STR profile of this cell line matches that of its parental cell line ATCC^® Catalog No. CCL-247. HCT-116 cancer cells are a human colorectal carcinoma cell line isolated from the colon of an adult male. The cells possess a near diploid karyotype and express carcinoembryonic antigen.

细胞系描述

These cells are a human colorectal carcinoma cell line isolated from the colon of an adult male. The cells possess a near diploid karyotype and express carcinoembryonic antigen.

应用

This product is a human HCT-116 carcinoma cell line in which a landing pad cassette has been integrated into the AAVS1 safe harbor locus using CompoZr^® Zinc Finger Nuclease technology. The mKATE2 fluorescence gene was integrated following the EF1a promoter and flanked by unique Cre-lox sites. The design of this landing pad cassette allows for easy, fast, and affordable genetic modification using Cre recombinase. mKATE2 can easily be exchanged for a payload of the user′s choice using Cre recombinase and a targeting vector with appropriate lox sites. Cells can then be sorted via fluorescence-activated cell sorting (FACS) for loss of mKATE2 expression as a surrogate for successful integration of the targeting vector. Approximately 7-10 days are required for loss of the mKATE2 signal in successfully targeted cells. See technical bulletin for detailed protocols.

特点和优势

These cells contain the mKATE2 fluorescence gene flanked by unique Cre-lox sites inserted in the AAVS1 safe harbor gene. These HCT-116 cells are adherent with a doubling time of approximately 22 hours.

质量

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

培养基

McCoy′s 5A medium modified to contain 1.5mM L-glutamine and 2200mg/L sodium bicarbonate. Add 10% FBS.

法律信息

ATCC is a registered trademark of American Type Culture Collection

CompoZr is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• 象形图: GHS05
• 警示用语：: Warning
• 危险声明: H290
• 预防措施声明: P234 - P390
• 危险分类: Met. Corr. 1
• 储存分类代码: 8A - Combustible, corrosive hazardous materials
• WGK: WGK 2
• 闪点(F): Not applicable
• 闪点(C): Not applicable