Merck
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CLLS1110

Sigma-Aldrich

MCF10A CELLS SMO W535L

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NACRES:
NA.81

生物来源

human male mammary glands (Source Disease: Fibrocystic Disease)

质量水平

200

OMIM登记号

601500

储存温度

−196°C

Gene Information

human ... SMO(6608)

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MCF10A CELLS SMO W535L

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MCF10A CELLS SYK (-/-)

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MCF10A CELLS EEF2K (-/-)

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MCF10A CELLS CDC25B (-/-)

biological source

human male mammary glands (Source Disease: Fibrocystic Disease)

biological source

human female mammary glands (Source Disease: Fibrocystic Disease)

biological source

human female mammary glands (Source Disease: Fibrocystic Disease)

biological source

human female mammary glands (Source Disease: Fibrocystic Disease)

OMIM accession no.

601500

OMIM accession no.

600085

OMIM accession no.

606968

OMIM accession no.

116949

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Gene Information

human ... SMO(6608)

Gene Information

human ... SYK(6850)

Gene Information

human ... EEF2K(29904)

Gene Information

human ... CDC25B(994)

一般描述

MCF10A CELLS SMO W535L are pre-neoplastic mammary epithelial cells from a human adult female with a ZFN knock in modification.
This cell line corresponds to ATCC Catalog No. CRL-10317.

应用

Cell freezing medium-DMSO 1X, Cat. No. C6164
Media renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°
The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat No. H6909, to a final concentration of 0.5 ug/mL.

特点和优势

These MCF10A cells are adherent with a doubling time of approx. 20 hours
Zinc finger nuclease (ZFN) knockin on chromosome 7q32.1

质量

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

Kit Components Also Available Separately

Product No.
Description
SDS
  • MCF10A CONTROL CELLS 1 vial

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