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CORET

Sigma-Aldrich

含有Taq DNA聚合酶的PCR核心试剂盒

PCR components in separate tubes to allow for optimization

eCl@ss:
32161000
NACRES:
NA.55

质量水平

200

形式

liquid

用途

sufficient for 250 reactions

特点

dNTPs included
hotstart: no

technique(s)

PCR: suitable

颜色

colorless

input

purified DNA

运输

wet ice

储存温度

−20°C

Related Categories

一般描述

Taq DNA聚合酶是一种从嗜热菌水生栖热菌( Thermus aquaticus)衍生的特殊热稳定酶。这种酶采用重组形式,在大肠杆菌中表达。Taq DNA聚合酶可以承受高达95 °C的高温而不明显丧失活性。Taq DNA聚合酶是一种依赖镁离子的酶。Taq DNA聚合酶拥有5′→3′ DNA聚合酶活性和5′→3′外切酶活性。

应用

CORET PCR试剂盒用于人心果不同物种遗传多样性研究的基因组DNA扩增。
带有Taq DNA聚合酶的PCR Core试剂盒适用于基因组DNA、cDNA或克隆DNA模板的扩增。每个成分都经过聚合酶链反应的测试。Taq聚合酶、模板DNA、引物和MgCl2的最佳浓度取决于系统的具体情况。可能需要对各组分分别进行优化,特别是Taq和Mg2+的浓度和循环参数。

数量

试剂盒中含有足够用于250单位Taq DNA聚合酶的试剂。

其他说明

以下试剂必需但并未提供在试剂盒内:
  • 矿物油,M 8662(可选)
  • 热循环仪
  • 引物
  • 待扩增的DNA
  • 氯仿,C 7559(可选)

法律信息

No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Kit Components Also Available Separately

Product No.
Description
SDS

  • P219210X PCR Buffer, Optimized for routine PCR with MgCl2 included 1 mL/vial

  • P231710X PCR Buffer without MgCl2, Optimized for routine PCR without MgCl2 1 mL/vial

  • M8787Magnesium chloride solution, PCR Reagent, 25 mM MgCI2 solution for PCR 1 mL/vial

  • W1754Water, PCR Reagent 3 vial(s)

  • D7295Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent .25 mL

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

SDS

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