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D1556

Sigma-Aldrich

OptiPrep密度梯度培养基

used for cell and subcellular organelle isolation

别名:
碘克沙醇 溶液
CAS号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.75

质量水平

200

应用

hematology: suitable
histology: suitable

SMILES string

CC(=O)N(CC(O)CN(C(C)=O)c1c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c1I)c2c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c2I

InChI

1S/C35H44I6N6O15/c1-13(52)46(30-26(38)20(32(59)42-3-15(54)9-48)24(36)21(27(30)39)33(60)43-4-16(55)10-49)7-19(58)8-47(14(2)53)31-28(40)22(34(61)44-5-17(56)11-50)25(37)23(29(31)41)35(62)45-6-18(57)12-51/h15-19,48-51,54-58H,3-12H2,1-2H3,(H,42,59)(H,43,60)(H,44,61)(H,45,62)

InChI key

NBQNWMBBSKPBAY-UHFFFAOYSA-N

应用

OptiPrep密度梯度培养基已用于从支气管肺泡灌洗液中进行无包膜病毒颗粒的分离。
密度梯度也适用于:
  • 细胞和细胞器分离
  • 病毒纯化
  • 脂蛋白和大分子分离

包装

250 mL in poly bottle

其他说明

60% (w/v) 碘克沙醇水溶液(无菌)。

法律信息

OptiPrep is a trademark of Serumwerk Bernburg AG

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Why choose Product D1556, OptiPrep, instead of other density gradients such as Percoll or Histopaque?

    Percoll and Histopaque must be removed prior to use through washing.  If Percoll and Histopaque are not removed, these density gradients can be toxic to mammalian cells.  In comparison, OptiPrep has no potential for bioaccumulation and can be considered nontoxic to cells. OptiPrep also has lower viscosity as compared to polysucrose based products such as Histopaque or Ficoll.

  4. What is the appearance of Product D1556, OptiPrep?

    The solution may be clear to slightly yellow.

  5. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  6. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  7. How do I store solutions of Product D1556, OptiPrep Density Gradient Medium?

    After diluting Product D1556, OptiPrep Density Gradient Medium, to the required density, the solution can be stored at 4°C. It should be mixed well before use to make sure it has not settled.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Erica A. S, et al.
Emerging Infectious Diseases, 21(1), 103-106 (2015)
Kiyoko Okamoto et al.
Journal of virology, 82(17), 8349-8361 (2008-06-20)
Hepatitis C virus (HCV) core protein has shown to be localized in the detergent-resistant membrane (DRM), which is distinct from the classical raft fraction including caveolin, although the biological significance of the DRM localization of the core protein has not...
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