Merck
All Photos(1)

D2906

Sigma-Aldrich

杜氏改良Eagle 培养基/营养混合物 F-12 Ham

With L-glutamine and 15 mM HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

All Photos(1)
Synonym(s):
DME/F-12, 1:1混合
MDL编号:
MFCD00217417
NACRES:
NA.75

质量水平

400

形式

powder

technique(s)

cell culture | mammalian: suitable

组分

NaHCO3: no
L-glutamine: yes
phenol red: no
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L
HEPES: 15 mM

运输

ambient

储存温度

2-8°C

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D8437D6421D8900
Sigma-Aldrich

Sigma-Aldrich

D2906

杜氏改良Eagle 培养基/营养混合物 F-12 Ham

Sigma-Aldrich

Sigma-Aldrich

D8437

杜氏改良Eagle 培养基/营养混合物 F-12 Ham

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

D6421

杜氏改良Eagle 培养基/营养混合物 F-12 Ham

Sigma-Aldrich

Sigma-Aldrich

D8900

杜氏改良Eagle 培养基/营养混合物 F-12 Ham

form

powder

form

liquid

form

liquid

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
L-glutamine: yes
phenol red: no
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L
HEPES: 15 mM

components

glucose: 3.15 g/L
HEPES: yes
L-glutamine: yes
phenol red: yes
sodium pyruvate: 0.055 g/L

components

sodium pyruvate: 0.055 g/L
L-glutamine: no
phenol red: yes
NaHCO3: yes
HEPES: 15 mM
glucose: 3.15 g/L

components

sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

500

一般描述

适用于在低血清条件下生长多种细胞类型。克隆和干细胞培养的研究者通常更喜欢去除酚红。

应用

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used to culture:

  • Hep-2 cells
  • mouse fibroblast L‐cells
  • L‐ Wnt family member 3A (Wnt3a) cells
  • primary buffalo embryonic fibroblast (BEF)

数量

配方中每升培养基含有15.6克的粉末。

重悬

并补充1.2 g/L碳酸氢钠。

储存分类代码

11 - Combustible Solids

WGK

WGK 1

Certificate of Analysis

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Certificate of Origin

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