Merck

D3669

Sigma-Aldrich

3,3′-二硫代二丙酸二(N-羟基丁二酰亚胺酯)

powder

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别名:
DTSP, Lomant’s试剂, 二(N-琥珀酰亚胺基)-3,3′-二硫代二丙酸酯, 二硫代双(琥珀酰亚胺丙酸酯)
Empirical Formula (Hill Notation):
C14H16N2O8S2
CAS号:
分子量:
404.42
Beilstein:
1518074
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NC.07

形式

powder

reaction suitability

reagent type: cross-linking reagent

溶解性

chloroform: 50 mg/mL
DMF: soluble
acetone: soluble

储存温度

−20°C

SMILES string

O=C(CCSSCCC(=O)ON1C(=O)CCC1=O)ON2C(=O)CCC2=O

InChI

1S/C14H16N2O8S2/c17-9-1-2-10(18)15(9)23-13(21)5-7-25-26-8-6-14(22)24-16-11(19)3-4-12(16)20/h1-8H2

InChI key

FXYPGCIGRDZWNR-UHFFFAOYSA-N

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P3415M5525M9794
reaction suitability

reagent type: cross-linking reagent

reaction suitability

reagent type: cross-linking reagent

reaction suitability

reagent type: linker

reaction suitability

reagent type: linker

solubility

chloroform: 50 mg/mL, DMF: soluble, acetone: soluble

solubility

acetone: 50 mg/mL, DMF: soluble

solubility

chloroform: 50 mg/mL, DMF: soluble

solubility

chloroform: 100 mg/mL, DMF: soluble (lit.)(lit.)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

一般描述

3,3'-二硫代二丙酸二(N-羟基琥珀酰亚胺酯)(DSP)是一种含有可裂解二硫键的同双功能交联剂。它通常在pH 7.5(6.5-8.5)的缓冲液中与含有伯胺的分子通过酰胺键发生偶联。

应用

3,3'-二硫代二丙酸二(N-羟基琥珀酰亚胺酯)(DSP)已被用作蛋白质交联剂。

特点和优势

通过温和还原,可使二硫键裂解。

注意

在偶联反应过程中避免使用还原剂。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves


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M Darder et al.
Analytical chemistry, 71(24), 5530-5537 (2000-01-07)
Exposure of gold surfaces to solutions of dithiobis N-succinimidyl propionate (DTSP) gives rise to the modification of the surface with N-succinimidyl-3-thiopropionate (NSTP) which can, in turn, react with amino groups allowing for the covalent immobilization of enzymes such as horseradish
Protein phosphatases 2C regulate the activation of the Snf1-related kinase OST1 by abscisic acid in Arabidopsis.
Vlad F
Plant Cell, 21, 3170-3170 (2009)
Sunil K Arya et al.
The Analyst, 136(3), 486-492 (2010-11-17)
A bioassay platform using T4 bacteriophage (T4) as the specific receptor and surface plasmon resonance (SPR) as the transduction technique has been developed for the detection of Escherichia coli K12 bacteria. The T4 phages have been covalently immobilized onto gold
Rui Deng et al.
Journal of controlled release : official journal of the Controlled Release Society, 140(1), 40-46 (2009-07-25)
The commercially available branched polyethyleneimine (PEI) with a molar mass of 25 kD (PEI-25K) is an effective in vitro vector to transfer genes, but its cytotoxicity limits its applications in bio-related research. To solve such an efficiency-versus-cytotoxicity catch-22 problem, the
N S Green et al.
Protein science : a publication of the Protein Society, 10(7), 1293-1304 (2001-06-23)
Homobifunctional chemical cross-linking reagents are important tools for functional and structural characterization of proteins. Accurate measures of the lengths of these molecules currently are not available, despite their widespread use. Stochastic dynamics calculations now provide quantitative measures of the lengths

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